Skip to main content

Table 1 The sequences of the forward and reverse primers flanking the promoter, 19 exons, and 3'flanking sequence for canine FGFR3 (Genbank Accession: EU853457), annealing temperatures, and amplicon sizes

From: Canine fibroblast growth factor receptor 3 sequence is conserved across dogs of divergent skeletal size

Ensembl Exon

Forward

Reverse

Anneal (°C)

Amplicon (bases)

Promotera

gacgcgtggcctagattc

gagcatgtgcccctgatac

60

699

1 and 2

caaacctcccagaacaggac

cccgcagggatacagtctt

61.5

833

3

cgtgtgcaggtgctcagtat

gtgtcctcagcctcatcctc

60

495

4

cgtgcgtgtgacaggtaaat

ctgcagtacaggtccccaac

59

394

5

accatgtggcttagccttga

tgtttctccacaacgcatgt

59

472

6

ccatctcgtggctgaagaac

gctgtacaccttgcagtgga

58

437

7

acatgcgttgtggagaacaa

taccacttctcccctgatgg

55

399

8 IIIbb

cagcatttctgactgcagga

ggctcggaacctggtatcta

58

401

8 IIIcc

atgtggactctggctgtggt

cacgagttctgtggagcaag

60

301

9

acacccccttctccattctc

gagtgcagtgcgagtctcag

59

407

10

tggagcctgggttatttgtc

cagtcatcacactgcccatc

59

500

11

aaggttgtggggcaagtatg

ccaggtctgagaggtccttg

59

358

12

aggccatcggtattgacaag

gtacaggggtcttggagcag

60

349

13

tccttcctgcacagatgatg

aagctcccaagtggtcctg

58

461

14

ttctccctccccccccttccccagac

tcccgagggcaggggcccttgtc

59

350

15

cagccaggccctggctgccgccac

agggcacctggccgtcaacatgc

59

394

16

accgagtctacacccaccag

acaatgcctcccatgacc

59

426

17

atggacaagccagccaact

ccgacaggtccagatactcc

60

401

18 and 3' flanking

gcagctagtggaggatctgg

cacaccaccagcagcatagt

60

361

  1. a. Amplicon represents the putative promoter region
  2. b. Exon indicating the FGFR3 IIIb isoform
  3. c. Exon indicating the FGFR3 IIIc isoform