Figure 1

Differential systemic movement of TMV-U1 in two Arabidopsis thaliana ecotypes. Col-0 and Uk-4 plants were mechanically inoculated on three rosette leaves with a solution of 10 ng/μl of virus diluted in 20 mM phosphate buffer. (a) In situ hybridisation of inoculated and apical leaves from infected plants at 10 dpi. Leaves were incubated with a TMV-U1 coat protein DNA probe. The virus coat protein gene was detected ubiquitously in inoculated leaves from both ecotypes, but only in an apical leaf of Uk-4 plants. Images represent equivalent levels of exposure to film. (b) Western blot showing similar time-courses of TMV-U1 infection in inoculated leaves of Col-0 (upper) and Uk-4 (bottom) plants. Coat protein is evident in both cases starting from 4 dpi. (c) ELISA showing differences in the systemic spread of TMV-U1 in Col-0 and Uk-4 plants. Apical leaves of inoculated plants were analysed for 18 dpi.