Flow cytometyric analyses of relationship of surface expression of CD14 on monocytes and neutrophils with CD14 genotypes. Cells were stained with fluorescein isothiocyanate (FITC)-labelled mouse anti human CD14 antibody and 20,000 events were gated and analyzed with Windows Multiple Document Interface for flow cytometry (WinMDI) software version 2.8. (a) Histogram showing a control sample that was not stained with antibody and occupies the Log100 to 101 region and known as the control zone. M1 (Log101 to 102) is the zone of low expression or lower fluorescence zone indicating lower number of CD14 antigens on cells, M2 (Log102 and higher) is the zone of higher fluorescence emitted by a higher rate of absorption by more CD14 antigens on cells and M3 is the total area of expression. (b) Histogram showing stained monocytes with a higher percentage of cells in M2 and a higher overall MCF intensity of 201.44 as compared to 34.76 for polymorphonuclear neutrophils; (c) Histogram showing stained polymorphonuclear neutrophils with a higher percentage of cells in M1.