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Table 1 Immune responses (mean ± SD) and analyses of variance in the group immunized with KLH

From: Correlated effects of selection for immunity in White Leghorn chicken lines on natural antibodies and specific antibody responses to KLH and M. butyricum

   Line
Measure Sample size ND3-L PHA-L CC-L Control
Trait under selection
   ND3 48 5.75 ± 1.65a 3.00 ± 1.54b 1.64 ± 1.06c 2.56 ± 1.34b
   PHA 48 1.13 ± 0.44c 1.97 ± 0.92a 1.34 ± 0.73c 1.68 ± 0.81b
   CC 48    0.30 ± 0.07a 0.17 ± 0.05b
Before immunization with KLH (d 0)
   KLH 50 3.89 ± 1.45 4.00 ± 1.22 3.78 ± 1.23 3.69 ± 1.09
   LPS 50 1.95 ± 1.73 1.85 ± 1.58 2.27 ± 1.93 1.99 ± 1.64
   LTA 50 4.90 ± 1.51a 4.84 ± 1.43a 4.08 ± 1.32b 3.93 ± 1.23b
   Anti-Gal 50 13.68 ± 3.82a 12.88 ± 3.03a 10.89 ± 2.92b 10.75 ± 2.46b
After immunization with KLH (d 7)
   KLH 50 8.95 ± 1.95 9.23 ± 2.24 8.04 ± 2.22 8.85 ± 2.06
   LPS 49 4.49 ± 1.85ab 4.08 ± 1.61bc 3.40 ± 2.15c 5.02 ± 2.32a
   LTA 48 6.12 ± 1.21a 5.93 ± 1.20a 5.17 ± 1.33b 5.44 ± 1.18b
   Anti-Gal 50 20.08 ± 5.22 18.97 ± 4.15 19.64 ± 3.82 18.50 ± 3.02
   Analysis of variance
   Line Sex Interaction R 2
Trait under selection
   ND3 48 *** ns ns 0.79
   PHA 48 *** ns ns 0.72
   CC 48 *** ns ns 0.83
Before immunization with KLH (d 0)
   KLH 50 ns ns * 0.55
   LPS 50 ns ns ns 0.53
   LTA 50 *** ns ns 0.64
   Anti-Gal 50 ** ns ns 0.76
After immunization with KLH (d 7)
   KLH 50 ns ns ns 0.71
   LPS 49 * * ns 0.71
   LTA 48 *** ns ns 0.65
   Anti-Gal 50 ns ** ns 0.76
Responsiveness (d 7-d 0)
   KLH 50 ns ns ns 0.72
   LPS 50 *** ns ns 0.75
   LTA 50 ns ns ns 0.73
   Anti-Gal 50 ns *** ns 0.75
  1. a-c: Means in the same row with no common superscript differed significantly (P ≤ 0.05) based on Duncan's multiple range test.
  2. 1 Antibody titers for to KLH, LPS and LTA are the log2 of the reciprocal of the antibody dilution.
  3. 2Antibody titers to anti-Gal epitope measured in plasma samples as the agglutination titer of glutaraldehyde stabilized rabbit. Titers are expressed as the double of the column number of the last plasma dilution showing clear evidence of agglutination. Responsiveness, measured as difference between the Ab titers at day 0 and day 7 post KLH immunization
  4. ND3-L = line selected for high antibody response to ND3 3 wk after vaccination.
  5. PHA-L = line selected for high cell-mediated immune response, to PHA at 9 wk of age.
  6. CC-L = line selected for high phagocytic activity, measured as carbon clearance at 12 wk of age.
  7. Control = line selected at random
  8. * P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.