Figure 4

Growth of the tra1 _{SRR 3413} strain under conditions of cellular stress. Yeast strains BY7092 (TRA1) and CY2222 (tra1_{SRR 3413}) were grown overnight to saturation in YPD at 30°C. 5 μl of 10-fold serial dilutions were spotted onto synthetic complete media containing 2% glucose (SC) or with the addition of 2 nM rapamycin, 2 μg/ml staurosporine (stauro), 7.5 μg/ml calcofluor white (CW), or 2 μg/ml staurosporine plus 7.5 μg/ml calcofluor white (CW stauro). Cells were grown for 3 days on SC and 5 days on selective plates, at 30°C.