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Figure 4 | BMC Genetics

Figure 4

From: Autosomal mutations affecting Y chromosome loops in Drosophila melanogaster

Figure 4

Wild type meiosis of D. melanogaster. Left column: immunostaining with anti α-tubulin; right column: Hoechst 33258 staining. a-b: early prometaphase I in wild type primary spermatocytes; note the prominent asters (a) and the three clumps of chromatin (b), corresponding to the major autosomes and the sex chromosomes; spaces between the clumps are filled by Y-loops. c-d: metaphase I; chromosome condensation is complete (d), and spindle fibers contact centromeres. e-f: anaphase I. g-h: telophase I; note the replicated asters (arrows in g) and the mitochondria migrating along the spindle (small white dots in h). i-j: metaphase II cells. k-l: spermatids at the onion stage; note the weak staining of nebenkern due to mitochondrial DNA and the 1:1 association of nuclei and nebenkern of similar size. Bar: 10 μm

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