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Figure 5 | BMC Genetics

Figure 5

From: GpnmbR 150Xallele must be present in bone marrow derived cells to mediate DBA/2J glaucoma

Figure 5

Tolerogenic capacities and expression profiles of Gpnmb deficient APCs. (A) OVA-pulsed APCs isolated from mice of the indicated genotypes were injected into B6D2F1/J recipients. Their ability to induce immune deviation was then measured. Absence of ear swelling indicated functional immune deviation through the suppression of a DTH response to the OVA challenge. We then tested their ability to induce immune deviation as detected by inhibition of DTH i.e. suppression of ear swelling in response to OVA challenge. The experimental summary for each group follows: Untreated (APCs cultured in media, injected into mice, immunization, challenge), TGFβ2-treated (APCs cultured in presence of TGFβ2, injected into mice, immunization, challenge), Neg. Control (challenge only). Induction of immune deviation is detected as the suppression of ear swelling response in the TGFβ2-treated groups as compared to the untreated group. Ear swelling responses are presented as mean ± SEM. (B-C) Gene expression profiles of APCs from indicated genotypes. Real-time PCR was employed to determine the expression levels of the indicated genes and expression of each gene was normalized to Gapdh. To faciliate visualization of the overall pattern of gene expression changes across these genes, the expression changes in response to TGFβ2-treatment are summarized with an arrow (indicating up or down regulation) or a dash (indicating no change). TSP, thrombospondin 1, official gene name Thbs1; TGFβ2, transforming growth factor, beta 2; TNFR2, tumor necrosis factor receptor superfamily, member 1b, official gene name Tnfrsf1b; CD40, CD40 antigen; IL-6, interleukin 6.

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