Figure 4
From: GpnmbR 150Xallele must be present in bone marrow derived cells to mediate DBA/2J glaucoma
GPNMB localizes to multiple pigmented tissues of the eye and ocular APCs. Cryosections of whole eyes from wild-type C57BL/6J mice were imaged in brightfield or phase illumination. GPNMB protein localization was imaged by fluorescent immunohistochemistry and confocal microscopy (red). GPNMB is present in: (A, B) multiple pigmented tissues of the eye, (C, D) iris and pigmented epithelia of the ciliary body, and (E, F) choroid. (G) Iris of normal C57BL/6J mouse showing GPNMB (red) within cells labeled by F4/80 (green). An 8 micron Z-series composite demonstrating F4/80 labeling the border of a single cell (dashed white circle) containing GPNMB. (H) BM derived dendritic cell from D2-Gpnmb+ marrow differentiated in cell culture showing punctate expression of GPNMB, (I, J) Negative controls were labelled and imaged with identical conditions and exhibited negligible signal. (I) Eyes from D2 mice (homozygous for GpnmbR 150X) have no signal for GPNMB, an inset on right hand corner shows the brightfield image of the same section. (J) BM derived dendritic cells from D2 mice have no staining for GPNMB. Scale bars A-F, I = 500 μm, G, H, J = 10 μm.