Figure 1

Gpnmb is a candidate for mediating BM derived contributions to D2 glaucoma. A). As expected for a candidate capable of mediating the BM derived contributions to the D2 form of glaucoma, Gpnmb is expressed in BM derived cells. Agarose gel of designated RT-PCR products amplified from iris (I), bone marrow (BM), lymph node (L), or thymus (T) of normal C57BL/6J mice. (B) Iris lysates from D2 and D2-Gpnmb⁺ mice were analyzed on Western blot with anti-GPNMB antibody (B). There is no protein of the expected size for GPNMB (arrow) in the D2 lysate. The faint bands of lower molecular weight likely represent background cross-reactivity of the antibody.