Figure 6

Tissue expression distribution of Bm-ASH genes in silkworm larvae. RT-PCR templates including: ectoderm tissues H (head), WD (wing disc), S-G (silk gland), MT (Malpighian tubule), TC (trachea cluster), BW (body wall); mesoderm tissues He (hemocyte), FB (fat body), G (gonad, T (testis), O (ovary)); and endoderm tissue MG (midgut). V3d stands for 3-day-old larvae of the 5th instar, and S stands for 8-day-old larvae of the 5th instar (begin spinning). Amplification cycles were 30 for Bm-ASH1, Bm-ASH2, Bm-ASH3 and Bm-ase, and 25 for the internal control gene Bm-actin A3.