A mitochondrial mutator plasmid that causes senescence under dietary restricted conditions

BMC Genomic Data

Table 1 Plasmid/mtDNA recombination junctions

#^a	Location^c	Position^d
01.	lrn-i1 (1x)	3,919–21
02.	orf p' (1x)	28,808–12
03.	nd1-i4 (1x)	98,017–25
04.	nd1-i4 (3x)	98,017–25
05.	between trna-cys and co1 (1x)	38,434–42
06.	nd3-i1 (1x)	14,362–67
07.	cob-i1 (1x)	32,164–70
08.	between orf c and atp6 (1x)	22,656–60
09.	between orf c and atp6 (1x)	22,979/80
10.	between orf c and atp6 (2x)	23,009/10
11.	pAL2-1 homologue, ddrp (3x)	3,021/22
12.	pAL2-1 homologue, ddrp (2x)	2,599/00
13.	orf p' (1x)	28,805/06
14.	between trna-met2 and nd2 (1x)	11,337–39
15.	between trna-met2 and nd2 (1x)	11,555–57

a. Four of the recombinant sequences (numbers 1, 5, 10 and 14) correspond to 3' plasmid/mtDNA recombination junctions; nine of them (numbers 2–4, 6–9, 13 and 15) to 5' plasmid/mtDNA recombination junctions. Note the MUSE1 undecamer (underlined) found at integration site number 10. Junctions from strain Wa30 (numbers 1–12) were found under DR conditions and analyzed by ST-PCR; those from Wa32 (numbers 13 and 14) were found under regular conditions; the one from Wa32-LL (number 15) had already been sequenced using regular methods [21].
b. Alignments of recombinant plasmid/mtDNA and corresponding wild-type sequences, with plasmid-derived parts (corresponding to the 3' terminus of the plasmid) indicated in grey and potential homology in dark grey.
c. Locations where the plasmid/mtDNA recombination junctions were found, with the number of times particular junctions were found indicated in brackets.
d. Positions correspond to the mitochondrial genome sequence of P. anserina laboratory strain s [23, GenBank: X55026] or to the sequence of the prototypic pAL2-1 plasmid [8, GenBank X60707].

ISSN: 2730-6844