Table 7 Primers used for GDF8 exon scanning and mutation detection
From: Evidence for multiple alleles effecting muscling and fatness at the Ovine GDF8 locus
Oligonucleotide | Region | Sequence (5' – 3') |
|---|---|---|
MSTN1-F | promoter-exon1 | TGAATCAGCTCACCCTTGACT |
MSTN1-R | promoter-exon1 | GCTGCTGTCATCTCTCTGGA |
MSTN2-F | exon 1 – intron 1 | GCCATAAAAATCCAAATCCTCA |
MSTN2-R | exon 1 – intron 1 | ACAAGCCAGCAGCTTGTTAAA |
MSTN3-F | intron 1 – exon 2 | TTGTCCCCAGGTAATTCAGG |
MSTN3-R | intron 1 – exon 2 | GCCTGGGTTCATGTCAAGTT |
MSTN4-F | exon 2 – intron 2 | AGTAAAGGCCCAACTGTGGA |
MSTN4-R | exon 2 – intron 2 | TTGGGTACAGGGCTACCATC |
MSTN5-F | intron 2 – exon 3 | TGGAGTTCGTCTTTCCAACC |
MSTN5-R | intron 2 – exon 3 | CCCATCCAAAAGCTTCAAAA |
MSTN6-F | exon 3 - 3' UTR | TTGGGCTTGATTGTGATGAG |
MSTN6-R | exon 3 - 3' UTR | CTTAAGTGACTGTAGCATACTC |
MSTN7-F | 3' UTR | AGTGGTCTTAAAACTCC |
MSTN7-R | 3' UTR | CAATTTATTTGATTAACAAAATCCTGA |
g-41C>A-F | g-41C>A | GTTTGGCTTGGCGTTACTCAA |
g-41C>A-R | g-41C>A | CAAAGATTTGCAGTTTTTGCATGGTTTT |
g-41A probe | g-41C>A | VIC-TTGCTCTTACTTCTTCC |
g-41C probe | g-41C>A | FAM-TGCTCTTACTGCTTCC |
g+4036A>C-F | g+4036A>C | GCAGTACTGGGTGGCAACTATTTTA |
g+4036A>C-R | g+4036A>C | ATTCTCCATTCACTTGGTCTTGTTCA |
g+4036A probe | g+4036A>C | VIC-TTAATATCCAAATTTCCC |
g+4036C probe | g+4036A>C | FAM-ATCCACATTTCCC |
g+6723G>A-F | g+6723G>A | GGTTCGTGATGGCTGTATAATGTGA |
g+6723G>A-R | g+6723G>A | GATTTCAGATAATAGAGTTAAATCATTTTGGTTTGCTT |
g+6723G probe | g+6723G>A | VIC-TTCAAATCTCAACGTTCCAT |
g+6723A probe | g+6723G>A | FAM-TCAAATCTCAACATTCCAT |
BM81124-F | microsatellite | GCTGTAAGAATCTTCATTAAGCACT |
BM81124-R | microsatellite | CCTGATACATGCTAAGGTTAAAAAC |
Bulge20-F | microsatellite | CAGCAGGTCTGTTGAAGTGTATCAG |
Bulge20-R | microsatellite | AGTGGTAGCATTCACAGGTAGCCAG |