Figure 2

Results of PCR assay for uaDf5 and wild-type mtDNA. (A) Each lane contains the PCR assay products from an individual worm taken arbitrarily from our laboratory stock, except the leftmost lane, which is a molecular size marker (lambda phage genome digested with Pst I). (B) The relationship between the percent of mtDNA molecules with the uaDf5 deletion in the template and the resulting percent of uaDf5 in the amplified products. The percentages of uaDf5 in the template reactions were 0, 2, 5, 10, 25, 50, 75, 90, 95, and 100. Three replicate reactions for each concentration were run. The plotted points represent the mean for the three reactions with the standard deviations shown as error bars.