NOD2 detection in coronary atherosclerotic lesions by immunofluorescence. Panel A depicts an overview of a coronary artery section with a plaque at low magnification (HE staining, original magnification ×5). The same section has been stained for NOD2 (FITC, green) and the macrophage marker CD68 (Cy3, red) in B. The asterisk shows the strong autofluorescence of the fragmented external elastic membrane, the arrows depict NOD2 positive macrophages in deeper layers of the arterial wall (original magnification, ×10). The two inserts on the lower right hand side show double positive cells for CD68+ and NOD2. Panel C :is a higher power view of NOD2+/CD68+ cells, which can be found in the vicinity of the vessel lumen in the ruptured plaque (original magnification, ×200.). The area of the micrograph corresponds to the box shown in B in an adjacent section. Panel D depicts a higher power view of NOD2 co-localization with CD68+ macrophages in inflammatory infiltrates in the intima. (original magnification, ×50). Arrowheads indicate double-positive cells. Pictures are representative for five patients and five controls (heart beating donors).