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Table 2 Yeast-2-Hybrid analysis of Ppa-MBD-2

From: Functional diversification of the nematode mbd2/3 gene between Pristionchus pacificus and Caenorhabditis elegans

Gene

-LTH+3AT1

LacZ

mbd-2

-

NA

dlc2,3

+

+

T07C4.102,3

+

+

bath-38 2,3

+

+

tag-260 2,3

+

+

hip-1 2,3

+

+

2 × No sim.2,3

+

+

  1. 1 The assay was performed on plates depleted in leucine, triptophane and histidine, supplemented with 50 mM 3 AT. 2 Results were confirmed by shifting prey and bait constructs. mbd-2, autoactivation control (pDEST-32-Ppa-mbd-2). 3 Interactors found in the screen of the cDNA library. dlc, dineyn light chain. T07C4.10, myosin heavy chain homolog. Bath-38, BTB/MATH/POZ domain similar to Spop. Tag-260, proteins similar to C. elegans tag-260, annotated as a predicted E3-ubiquitin ligase. Hip-1, Huntingtin interacting protein, 2 × No sim, 2 clones with no homolog in the database. NA, not-applicable.