TY - JOUR AU - Ekstrøm, Per O. AU - Bjørheim, Jens AU - Thilly, William G. PY - 2007 DA - 2007/08/14 TI - Technology to accelerate pangenomic scanning for unknown point mutations in exonic sequences: cycling temperature capillary electrophoresis (CTCE) JO - BMC Genetics SP - 54 VL - 8 IS - 1 AB - Rapid means to discover and enumerate unknown mutations in the exons of human genes on a pangenomic scale are needed to discover the genes carrying inherited risk for common diseases or the genes in which somatic mutations are required for clonal diseases such as atherosclerosis and cancers. The method of constant denaturing capillary electrophoresis (CDCE) permitted sensitive detection and enumeration of unknown point mutations but labor-intensive optimization procedures for each exonic sequence made it impractical for application at a pangenomic scale. SN - 1471-2156 UR - https://doi.org/10.1186/1471-2156-8-54 DO - 10.1186/1471-2156-8-54 ID - Ekstrøm2007 ER -