Figure 6

Implementation of complementation strategy for rad2. For each, Lanes 1–20 contain product obtained from individual PCR reactions using total genomic DNA extracted from 20 individual Foa^r colonies as template and primers which reside ~500 bp 5' and 500 bp 3' of the rad2 gene targeted for deletion. A. PCR assay to screen for knockout alleles of flap endonuclease rad2 gene in Halobacterium sp. NRC-1. B. PCR assay to screen for knockout alleles of flap endonuclease rad2 gene in Halobacterium sp. NRC-1 derivatives transformed with a replicating plasmid containing a wild-type copy of the rad2 gene plus the entire 5' intergenic region. Both the wild-type and deletion alleles are observed.