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Table 3 Segregation efficiency in axenic medium and on bacterial plates.

From: Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics

Segregation Conditions Proportion Diploid Proportion Haploid Proportion Haploid Replacement
Axenic 147/152 (96.7%) 5/152 (3.3%) 2/152 (1.3%) [2/5 (40%)]
Bacterial Plates 3/36 (8.3%) 10/36 (27.8%) 6/36 (16.7%) [6/10 (60%)]
  1. Cells were segregated in axenic medium as described in Materials and Methods. Numbers are representative of three experiments.
  2. Cells were segregated on bacterial plates as described in Materials and Methods. Numbers are representative of two experiments.
  3. Numbers represent all haploids i.e. haploids with extra tagged copy of Arp2 and haploid replacements.
  4. Numbers reflect haploid replacements expressed as a proportion of the total number of haploids ().