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Table 2 Growth conditions of cell lines used in this work.

From: Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics

Strain

Growth Conditions

HM-1:IMSS (clone 9)

Axenic medium LYI-S-2

AX3

Axenic medium HL-5

DH1

HL-5 supplemented with Uracil

JH10

HL-5 supplemented with Thymidine

DIR1

HL-5

DJK45

HL-5 (blasticidin optional)

DMZ10

HL-5 supplemented with G418 (blasticidin optional)

DMZ11

HL-5 supplemented with G418 (blasticidin optional)

MZ10

HL-5 supplemented with Thymidine and G418 (blasticidin optional)

MZ11

HL-5 supplemented with Thymidine and G418 (blasticidin optional)