Strain | Parent(s) | Description | Genotype | Ref. |
---|---|---|---|---|
HM-1:IMSS (clone 9) | HM-1:IMSS | E. histolytica Lab strain | Wild type | [49] |
AX3 | NC4 | D. discoideum Lab strain | Wild type | [44] |
DH1 | AX3 | Uracil auxotroph | pyr 56Δthy Awt | [50] |
JH10 | AX3 | Thymidine auxotroph | pyr 56- thy A::pyr 56 | [51] |
DIR1 | DH1/JH10 | Diploid between DH1 & JH10 (thy A and pyr 56 alleles complemented – no dependence; Arp2 homozygote) | pyr 56-/Δ thy A::pyr 56/wt | [17] |
DJK45 | DIR1 | Arp2 heterozygous diploid (bsrr inserted in one copy of Arp2) | pyr 56-/Δ thy A::pyr 56/wt arp B-bsr/wt | This work |
DMZ10 | DJK45 + pJSK19 | Arp2 heterozygote expressing myc- tagged replacement Dictyostelium Arp2 | pyr56-/Δ thyA::pyr56/wt arp B-bsr/wt+ [myc Ddarp BpRHI76] | This work |
DMZ11 | DJK45 + pMZ12 | Arp2 heterozygote expressing myc- tagged replacement Entamoeba Arp2 | pyr 56-/Δ thy A::pyr 56/wt arp B-bsr/wt+ [myc Eharp BpRHI76] | This work |
MZ10 | DMZ10 | Thymidine dependent haploid rescued with replacement Dictyostelium Arp2 | pyr 56- thy A::pyr 56 arp B-bsr+ [myc Ddarp BpRHI76] | This work |
MZ11 | DMZ11 | Thymidine dependent haploid rescued with replacement Entamoeba Arp2 | pyr 56- thy A::pyr 56 arp B-bsr+ [myc Eharp BpRHI76] | This work |