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Table 1 Cell lines used in this work.

From: Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics

Strain Parent(s) Description Genotype Ref.
HM-1:IMSS (clone 9) HM-1:IMSS E. histolytica Lab strain Wild type [49]
AX3 NC4 D. discoideum Lab strain Wild type [44]
DH1 AX3 Uracil auxotroph pyr 56Δthy Awt [50]
JH10 AX3 Thymidine auxotroph pyr 56- thy A::pyr 56 [51]
DIR1 DH1/JH10 Diploid between DH1 & JH10 (thy A and pyr 56 alleles complemented – no dependence; Arp2 homozygote) pyr 56-/Δ thy A::pyr 56/wt [17]
DJK45 DIR1 Arp2 heterozygous diploid (bsrr inserted in one copy of Arp2) pyr 56-/Δ thy A::pyr 56/wt arp B-bsr/wt This work
DMZ10 DJK45 + pJSK19 Arp2 heterozygote expressing myc- tagged replacement Dictyostelium Arp2 pyr56-/Δ thyA::pyr56/wt arp B-bsr/wt+ [myc Ddarp BpRHI76] This work
DMZ11 DJK45 + pMZ12 Arp2 heterozygote expressing myc- tagged replacement Entamoeba Arp2 pyr 56-/Δ thy A::pyr 56/wt arp B-bsr/wt+ [myc Eharp BpRHI76] This work
MZ10 DMZ10 Thymidine dependent haploid rescued with replacement Dictyostelium Arp2 pyr 56- thy A::pyr 56 arp B-bsr+ [myc Ddarp BpRHI76] This work
MZ11 DMZ11 Thymidine dependent haploid rescued with replacement Entamoeba Arp2 pyr 56- thy A::pyr 56 arp B-bsr+ [myc Eharp BpRHI76] This work