Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics

BMC Genomic Data

Table 1 Cell lines used in this work.

Strain	Parent(s)	Description	Genotype	Ref.
HM-1:IMSS (clone 9)	HM-1:IMSS	E. histolytica Lab strain	Wild type	[49]
AX3	NC4	D. discoideum Lab strain	Wild type	[44]
DH1	AX3	Uracil auxotroph	pyr 56Δthy A^wt	[50]
JH10	AX3	Thymidine auxotroph	pyr 56^- thy A::pyr 56	[51]
DIR1	DH1/JH10	Diploid between DH1 & JH10 (thy A and pyr 56 alleles complemented – no dependence; Arp2 homozygote)	pyr 56^-/Δ thy A::pyr 56/^wt	[17]
DJK45	DIR1	Arp2 heterozygous diploid (bsr^r inserted in one copy of Arp2)	pyr 56^-/Δ thy A::pyr 56/^wt arp B^-bsr/wt	This work
DMZ10	DJK45 + pJSK19	Arp2 heterozygote expressing myc- tagged replacement Dictyostelium Arp2	pyr56^-/Δ thyA::pyr56/^wt arp B^-bsr/wt+ [myc Ddarp BpRHI76]	This work
DMZ11	DJK45 + pMZ12	Arp2 heterozygote expressing myc- tagged replacement Entamoeba Arp2	pyr 56^-/Δ thy A::pyr 56/^wt arp B^-bsr/wt+ [myc Eharp BpRHI76]	This work
MZ10	DMZ10	Thymidine dependent haploid rescued with replacement Dictyostelium Arp2	pyr 56^- thy A::pyr 56 arp B^-bsr+ [myc Ddarp BpRHI76]	This work
MZ11	DMZ11	Thymidine dependent haploid rescued with replacement Entamoeba Arp2	pyr 56^- thy A::pyr 56 arp B^-bsr+ [myc Eharp BpRHI76]	This work

ISSN: 2730-6844