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Table 1 Cell lines used in this work.

From: Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics

Strain

Parent(s)

Description

Genotype

Ref.

HM-1:IMSS (clone 9)

HM-1:IMSS

E. histolytica Lab strain

Wild type

[49]

AX3

NC4

D. discoideum Lab strain

Wild type

[44]

DH1

AX3

Uracil auxotroph

pyr 56Δthy Awt

[50]

JH10

AX3

Thymidine auxotroph

pyr 56- thy A::pyr 56

[51]

DIR1

DH1/JH10

Diploid between DH1 & JH10 (thy A and pyr 56 alleles complemented – no dependence; Arp2 homozygote)

pyr 56-/Δ thy A::pyr 56/wt

[17]

DJK45

DIR1

Arp2 heterozygous diploid (bsrr inserted in one copy of Arp2)

pyr 56-/Δ thy A::pyr 56/wt arp B-bsr/wt

This work

DMZ10

DJK45 + pJSK19

Arp2 heterozygote expressing myc- tagged replacement Dictyostelium Arp2

pyr56-/Δ thyA::pyr56/wt arp B-bsr/wt+ [myc Ddarp BpRHI76]

This work

DMZ11

DJK45 + pMZ12

Arp2 heterozygote expressing myc- tagged replacement Entamoeba Arp2

pyr 56-/Δ thy A::pyr 56/wt arp B-bsr/wt+ [myc Eharp BpRHI76]

This work

MZ10

DMZ10

Thymidine dependent haploid rescued with replacement Dictyostelium Arp2

pyr 56- thy A::pyr 56 arp B-bsr+ [myc Ddarp BpRHI76]

This work

MZ11

DMZ11

Thymidine dependent haploid rescued with replacement Entamoeba Arp2

pyr 56- thy A::pyr 56 arp B-bsr+ [myc Eharp BpRHI76]

This work