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Figure 4 | BMC Genetics

Figure 4

From: Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics

Figure 4

Immunoprecipitation. Washed, pelleted beads with bound mAb 9E10 and immunoprecipitated proteins were analysed by SDS-PAGE and western blotting as described in Materials and Methods. Cell lysates (L) and immunoprecipitates (IP) from three Dictyostelium cell types were examined; AX3 (wild type), MZ10 (haploid rescued with myc-tagged Dictyostelium Arp2 replacement), MZ11 (haploid rescued with myc-tagged Entamoeba Arp2 replacement). Blots were probed with antibodies to Dictyostelium Arp2, Arp3 and the p21-arc subunits.

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