Gene replacement using parasexual genetics. (A) Schematic representation of the method. (B) A scheme showing the genetics underlying the gene replacement method. (C) Western hybridisation screen for the gene replacement method. Banding patterns seen with Dictyostelium anti-Arp2 and mAb 9E10 anti-myc antibody are shown. Lane 1 shows the pattern seen for strains DH1, JH10, DIR1 and DJK45; lane 2 shows the pattern seen for strains DMZ10 and DMZ11; lane 3 shows the pattern for a haploid strain containing both wild-type and myc-tagged Arp2; lane 4 shows the pattern of the replacement strains MZ10 and MZ11.