Primers | Forward 5'-3' | Reverse 5'-3' | TM |
---|---|---|---|
1 | atgcaacagggagaaagagc | gaccagcattggtagcaggt | 57°C |
2 | tggacagcacggctctaaat | tggacagcacggctctaaat | 58°C |
3 | ggtctggtgatgacagttgtg | ggaagggaggcatgtcagta | 60°C |
4 | tgtattttagtacagacggggtttc | tggtgcctgattatgcagag | 60°C |
5 | gcttagcagtgcctgtccat | gccaaattgctgcctattct | 58°C |
6 | agggtggaagatctcagaag | caaagcgctgggattatg | 57°C |
7 | gctgaggcaggagaatcact | ttggccacccaacagttaat | 59°C |
8 | aaataaaaaggaagaagaagaaga | ccacccaacagttaataccta | 57°C |
9 | ggcagcaatggaattgaaag | cccacagtgctgggattac | 58°C |
10 | ccaggggtttgcagttacag | gcttattcactaggaccaagcac | 57°C |
11 | ttgcagtgagccaaaatcc | tcacccatcatgcaaaacat | 63°C |
12 | tgacctcaggtgatccactc | ggtgaaactgagggagtttg | 62°C |
13 | tgagaccctctccagtttgc | agacgaagaggtgggaacag | 60°C |
14 | tcacatcttacaaactcaacaaaaa | ccaggagggttgcttaggta | 59°C |
15 | ccagtctggcctcgagaact | tcacaaatattggccaggtg | 59°C |
16 | ccagtcctgtaggtgctaaa | cttagccctgaaggaaaaat | 56°C |
17 | tctaaatcactagcatttcctg | gaaagcattccgtaagatgt | 58°C |
18 | tgggttcagaagttcttctcacta | ccccataccctccttgctac | 62°C |
19 | gccagagcaggaagagtc | cgttgccactttgtttatc | 56°C |
20 | gctactcactaaatgttcagctcct | cttgttcccccaggtgact | 62°C |
21 | ctccaccggacttttggtaa | ggtgctcttctgttgccatt | 57°C |
22 | gaattgacccctcaaggaca | aggcagaggtttcagtgagc | 61°C |
23 | ggcagagttttgctcttgtca | tggtgaaaccccgtctctac | 58°C |
24 | agtgcaggggcaggatct | gcatggggacagaggaataa | 58°C |
25 | ccttttctcttcattttcccatt | ggatgagaggaaggaaagattc | 59°C |
26 | tgcctgctttagaatgttttcc | agacaggtgcgtggataagg | 59°C |
27 | aagttttcataagcacacttctaacc | tctctccagacaccagaagg | 61°C |