Table 3 LMW-GS gene specific primers designed based on consensus sequences of full-length LMW-GS cDNA clones and a genomic clone.
Primer | Primer sequence | Contig no.a | Tm (°C) | Product size (bp) |
|---|---|---|---|---|
LMWF3 | GACAAGTGCCATTGCACAGA | 5, 3 | 60 | |
LMWR4 | GCTGTACAACGGCACATTGA | 5, 3 | 60 | 820 |
LMWF10 | TCACAGCAACAACAACCACA | 7 | 60 | |
LMWR9 | CTATCTGGTGTGGCTGCAAA | 7 | 60 | 784 |
LMWF11 | CCAAACTCGGTTGCAAAAGT | AY542898 | 60 | |
LMWR11 | TGGTGGTTGTTGCGGTAGTA | AY542898 | 60 | 805 |