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Table 1 Number of BAC clones producing single amplicons using three primer pairs alone or in combination.

From: Hemi-nested touchdown PCR combined with primer-template mismatch PCR for rapid isolation and sequencing of low molecular weight glutenin subunit gene family from a hexaploid wheat BAC library

Primer pair(s)

Number of BAC clones producing single amplicons

LMWF3/LMWR4 (PP1)

16

LMWF10/LMWR9 (PP2)

8

LMWF11/LMWR11 (PP3)

7

PP1 and PP2

4

PP1 and PP3

22

PP2 and PP3

0

PP1, PP2 and PP3

15

Total

72