From: Hemi-nested touchdown PCR combined with primer-template mismatch PCR for rapid isolation and sequencing of low molecular weight glutenin subunit gene family from a hexaploid wheat BAC library
Primer pair(s)
Number of BAC clones producing single amplicons
LMWF3/LMWR4 (PP1)
16
LMWF10/LMWR9 (PP2)
8
LMWF11/LMWR11 (PP3)
7
PP1 and PP2
4
PP1 and PP3
22
PP2 and PP3
0
PP1, PP2 and PP3
15
Total
72