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Table 1 Number of BAC clones producing single amplicons using three primer pairs alone or in combination.

From: Hemi-nested touchdown PCR combined with primer-template mismatch PCR for rapid isolation and sequencing of low molecular weight glutenin subunit gene family from a hexaploid wheat BAC library

Primer pair(s) Number of BAC clones producing single amplicons
LMWF3/LMWR4 (PP1) 16
LMWF10/LMWR9 (PP2) 8
LMWF11/LMWR11 (PP3) 7
PP1 and PP2 4
PP1 and PP3 22
PP2 and PP3 0
PP1, PP2 and PP3 15
Total 72