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Figure 3 | BMC Genetics

Figure 3

From: Hemi-nested touchdown PCR combined with primer-template mismatch PCR for rapid isolation and sequencing of low molecular weight glutenin subunit gene family from a hexaploid wheat BAC library

Figure 3

Comparison of standard PCR with hemi-nested touchdown (TD) PCR. (A) Standard PCR with primers LMWF38d/R19b and annealing temperature of 50°C, (B) the first round TD PCR using primers LMWF38d/R19b and (C) the second round hemi-nested TD PCR using primers LMWF38d/R19c. In the TD program, the annealing temperature is progressively lowered from 65°C to 50°C by 0.5°C every cycle, followed by 15 additional cycles at 50°C. M, 1 kb plus DNA ladder; lanes 1–7, PCR amplification of TaE0879B17, TaE0877F13, TaE0742B23, TaE0469B11, TaE0428D3, TaE0356P04 and TaE0212J01, respectively.

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BMC Genomic Data

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