The separate and combined effects of MHC genotype, parasite clone, and host gender on the course of malaria in mice

Table 2 The effect of MHC type and MHC heterozygosity on the time course of disease symptoms in males.

	Parasitemia			Blood cell counts			Body weight change
	F	d.f.	P	F	d.f.	P	F	d.f.	P
Between subjects
MHC type	3.7	4, 79	0.009	4.4	4, 78	0.003	0.8	4, 78	0.53
MHC heterozygosity	0.2	1, 79	0.65	0.8	1, 78	0.39	0.1	1, 78	0.82
Within subjects (repeated measurements on individual mice)
Time	121.2	5, 75	<0.0001	77.4	10, 69	<0.0001	21.7	18, 61	<0.0001
Time × MHC	1.8	20, 249.7	0.018	0.8	40, 263.5	0.77	1.0	72, 242.2	0.53
Time × heterozygosity	0.9	5, 75	0.48	1.0	10, 69	0.47	1.4	18, 61	0.15

Nested ANOVA incorporating the fixed effect factors "MHC-heterozygosity" and "MHC type" (nested in "heterozygosity"), with repeated measures each of the same disease symptoms as in Table 1. For within-subject analyses we used the multivariate F-tests or Wilk's lambda when a factor had more than two levels as in "MHC". The table combines our findings on the genotypes H-2^ak, H-2^bk, and H-2^kk with the data of Wedekind et al. [30] who used exactly the same methods on the host genotypes H-2^aa, H-2^bb, and H-2^ab. This results in six MHC genotypes, three homozygous ones and three heterozygous ones. The analyses are separated for males (here) and females (Table 3) because the group H-2^kk was not represented in all experimental cells (a prerequisite for full-factorial analyses).

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