From: A missense mutation in PMEL17 is associated with the Silver coat color in the horse
Primer sequence | Forward primer (5'-3') | Reverse primer (5'-3') |
---|---|---|
5'UTR-Intron1 | GGATCCCTTGTCAGTTTTGC | AGGAGAGGAAAAACCAGAGC |
Exon 1-Exon 2 (LR) | GATGGATCCAGTGTCAGAGATG | ACTCTGGATACAGCTGCCTGTT |
Intron 1-Exon 2 | CGTGGGATGACGTTATCTTCT | AGTCAGGCCCCTGAATTTCT |
Exon 2–3 | GTCTCAAGGCAGCTCAGGAA | CATTGATGATGGTGTTGTTGG |
Exon 3–4 (LR) | CTACACTGGTTGGGGCAAAT | TCTGAGACAGAGGGCCAGAT |
Exon 3-Intron 3 | GGTGGCCCTGAAGATCAGTA | AGGGAATTGGAGCCCTTAGA |
Intron 3-Exon 4 | CTCTCTGGGAGCCGTGTTAG | CCCAGGTCTTCCAGACGTAA |
Exon 4-Intron 4 | TCCCCAGGAACCTGATGATA | CTTCAGAGGTGGGACCAGAG |
Exon 4–5 | TCCCCAGGAACCTGATGATA | GCGGTGGTAGACAGTCACTT |
Exon 5–6 | AGTGTCGGGGCTGAGCAT | CAGGCCACAGCTTGTCTTTT |
Exon 5–6(2) | TGCCCCTCGCTCACTCCCGCTCAGCCT | CATGAATGGGCTGGCATCTGGA |
Exon 6–7 | GGTAACGGTACAGAGTTGGTGGAA | GGACGATGTCCAGAGTGAGGGA |
Exon 6–7(2) | AGGTGCCAACTGCAGAGC | GGACGATGTCCAGAGTGAGG |
Exon 7-Intron 8 | ATGGCACAGCCACCTTATTC | GAAAGGTGTCAGTTTAGGTCAGG |
Exon 7–10 | CCAGAGCCCCCTGCTGGATGG | TATATCAGAGATGCAAGCACCATA |
Exon 9-Intron 10 | AATGTGTCTTTGGCTGATGC | TCTGCCCCTCTTACAGGTGA |
Intron 10-Exon 11 | GCAGGGAAGCTTGTAGAGTGA | CTCTCACCAAAGGGGGAAG |
Exon 10–11 | AGAGGCAGGCCTTGGGCAG | TGCTCTCACCAAAGGGGGAAG |
Exon 11–3'UTR | CAGGCGCAGACTTATGAAGC | AGGGAAGWCTGSRGRAAABA |
Pyroseq. PCR primers, Ex 11 | Biotin-TCCATTGCTTACCAGTTTCCTT | CTCACCAAAGGGGGAAGAG |
Pyroseq. Seq primer, Ex 11 | - | GCCCTGCTTCATAAGTCTG |
Pyroseq. PCR primers, Int.9 | CATGCCTGGTAGGTACTTGGA | Biotin-CCTCTTGACCTGTGAGCAGA |
Pyroseq. Seq primer, Int 9. | GGGGAGTGGGCAGAGGCT | - |
RACE primer | - | CCGGAGGGCAAAGGTCAGAGGTTG |