Figure 8
From: Loss of imprinting at the Dlk1-Gtl2 locus caused by insertional mutagenesis in the Gtl2 5' region
Meg8 / Rian imprinting analysis in Gtl2Δ5'Neo embryos. A) Imprinting assay for the Meg8 (left) and Rian (right) genes in Gtl2Δ5'NeoPat129 and Gtl2Δ5'Pat129 embryos. B) Imprinting assay for the Meg8 (left) and Rian (right) genes in Gtl2Δ5'NeoMat129 and Gtl2Δ5'Mat129 embryos. The Meg8 imprinting assay detects an Nla III polymorphism, with the site present in C mice and absent in D mice. The Rian imprinting assay detects a Hinf I polymorphism, with the site present in D mice, and absent in C mice.