PCR amplification of DNA polymorphisms from aged sunfish tissues, employing Lma20 primers to amplify microsatellite regions. Lane 1: molecular weight markers (Hi-Lo Marker, Minnesota Molecular), size indicated in basepairs. Lanes 2, 4, 6, 8, and 10: PCR amplification of sunfish DNA from tissues that were stored in 100% ethanol. Lanes 3, 5, 7, 9, and 11: PCR amplification of sunfish DNA from tissues that were stored in a dried state. The period of aging is indicated for each set of samples. 2% agarose gel stained with ethidium bromide.