PCR products of bluegill DNA (Lake Wapalanne), employing Lma20 primers to amplify microsatellite regions. Lane 1: molecular weight markers (Hi-Lo Marker, Minnesota Molecular), size indicated in basepairs. Lanes 2–7: bluegill Lma20 microsatellite polymorphisms. Buccal tissues employed for this experiment were stored overnight in 100% ethanol before DNA extraction after 24 hours. Heterozygotes and homozygotes for the Lma20 marker are clearly delineated in the individual fish. 2% agarose gel stained with ethidium bromide. The image was inverted to a negative by Scion computer software (Scion, Inc., Frederick, Maryland).