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Table 1 Fragment size corresponding to different β-lactoglobulin genotypes after digestion of a 252 bp PCR product with Hae III restriction enzyme.

From: Genotyping of β-Lactoglobulin gene by PCR-RFLP in Sahiwal and Tharparkar cattle breeds

Genotype

Fragment size after digestion with Hae III restriction enzyme

No. of genotypes

Uncut PCR Product

252 bp

Sahiwal (n = 228)

Tharparkar (n = 86)

A/A

144 bp and 108 bp

7

2

A/B

144 bp, 108 bp, 74 bp and 70 bp

63

63

B/B

108 bp, 74 bp and 70 bp

158

21