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Table 1 Fragment size corresponding to different β-lactoglobulin genotypes after digestion of a 252 bp PCR product with Hae III restriction enzyme.

From: Genotyping of β-Lactoglobulin gene by PCR-RFLP in Sahiwal and Tharparkar cattle breeds

Genotype Fragment size after digestion with Hae III restriction enzyme No. of genotypes
Uncut PCR Product 252 bp Sahiwal (n = 228) Tharparkar (n = 86)
A/A 144 bp and 108 bp 7 2
A/B 144 bp, 108 bp, 74 bp and 70 bp 63 63
B/B 108 bp, 74 bp and 70 bp 158 21