Table 4 Oligonucleotide sequences of capture probe P1, detection probe P2, synthetic targets, and PCR primers.
From: Linkage disequilibrium blocks, haplotype structure, and htSNPs of human CYP7A1 gene
SNP | Oligonucleotide sequence |
|---|---|
rs10504255 P1-A | ALD-AAAAAAAAAAAACCTGAGCACTAGCCAGCTGTGTTCTCAATTCTGTGTGTAA |
rs10504255 P1-G | ALD-AAAAAAAAAAAACCTGAGCACTAGCCAGCTGTGTTCTCAATTCTGTGTGTAG |
rs10504255 P2 | Phosphate-CTCTTTCCCAATATTTCAAT-biotin |
rs10504255 Target for P1-A | ATTGAAATATTGGGAAAGAGTTACACACAGAATTGAGAACA |
rs10504255 Target for P1-G | ATTGAAATATTGGGAAAGAGCTACACACAGAATTGAGAACA |
rs10504255 Forward | TAAACCTGCCTTGTCACGC |
rs10504255 Reverse | AGTTGCAAACGCTGGTTG |
rs8192879 P1-C | ALD-AAAAAAAAAAGAAAAAACAATCTGCCAATTAGAATACATATCTTTTCTTC |
rs8192879 P1-T | ALD-AAAAAAAAAAGAAAAAACAATCTGCCAATTAGAATACATATCTTTTCTTT |
rs8192879 P2 | Phosphate-GGAGACGGGATCTCACTAAG-biotin |
rs8192879 Target for P1-C | CTTAGTGAGATCCCGTCTCCGAAGAAAAGATATGTATTCTA |
rs8192879 Target for P1-T | CTTAGTGAGATCCCGTCTCCAAAGAAAAGATATGTATTCTA |
rs8192879 Forward | ACCTGTAGTCTTAGCTACTCG |
rs8192879 Reverse | CCTTAGGAAAAAACAATCTGCC |
rs11786580 P1-C | ALD-AAAAAAAAAACATTTACAAGAACCTATTTTTATCCATATACTATCTGATGC |
rs11786580 P1-T | ALD-AAAAAAAAAACATTTACAAGAACCTATTTTTATCCATATACTATCTGATGT |
rs11786580 P2 | Phosphate-TGCGTGGCGATCACCGCTGC-biotin |
rs11786580 Target for P1-C | GCAGCGGTGATCGCCACGCAGCATCAGATAGTATATGGATA |
rs11786580 Target for P1-T | GCAGCGGTGATCGCCACGCAACATCAGATAGTATATGGATA |
rs11786580 Forward | TCTAGGTGTTTAATGCAGCTTC |
rs11786580 Reverse | GTCCCTTTATGCCTTTACCG |
rs8192874 P1-C | ALD-AAAAAAAAAATTCTCGTGCCTCAAGCTCTCTGCCAGTTTCTCCCGGGCAC |
rs8192874 P1-T | ALD-AAAAAAAAAATTCTCGTGCCTCAAGCTCTCTGCCAGTTTCTCCCGGGCAT |
rs8192874 P2 | Phosphate-TGTGCGCAGTCCTGAACATG-biotin |
rs8192874 Target for P1-C | CATGTTCAGGACTGCGCACAGTGCCCGGGAGAAACTGGCAG |
rs8192874 Target for P1-T | CATGTTCAGGACTGCGCACAATGCCCGGGAGAAACTGGCAG |
rs8192874 Forward | TCAGTTCTGAGATGCTTTCCC |
rs8192874 Reverse | AGTCTTTCCAGCCCTGGTAG |
rs2162459 P1-A | ALD-AAAAAAAAAATCTCTAGAGGTGGTTCACCCGTTTGCCTGTCAGACACAAA |
rs2162459 P1-G | ALD-AAAAAAAAAATCTCTAGAGGTGGTTCACCCGTTTGCCTGTCAGACACAAG |
rs2162459 P2 | Phosphate-TGTATGATAGACATGGATGA-biotin |
rs2162459 Target for P1-A | TCATCCATGTCTATCATACATTTGTGTCTGACAGGCAAACG |
rs2162459 Target for P1-G | TCATCCATGTCTATCATACACTTGTGTCTGACAGGCAAACG |
rs2162459 Forward | AAATTGCAGAGCACAGCC |
rs2162459 Reverse | TCAAAGTTTGAAGTCAGTGGG |
rs3808607 P1-G | ALD-AAAAAAAAAACAAAGCAATCAGAGACCTGCAATACTTGATAAGTTGAAGG |
rs3808607 P1-T | ALD-AAAAAAAAAACAAAGCAATCAGAGACCTGCAATACTTGATAAGTTGAAGT |
rs3808607 P2 | Phosphate-TCTCTCAAATATATGTTGAC-biotin |
rs3808607 Target for P1-G | GTCAACATATATTTGAGAGACCTTCAACTTATCAAGTATTG |
rs3808607 Target for P1-T | GTCAACATATATTTGAGAGAACTTCAACTTATCAAGTATTG |
rs3808607 Forward | AGTCCACAGGTATCAGAAGTG |
rs3808607 Reverse | CCCCAGGTCCGAATGTTAAG |
rs7833904 P1-A | ALD-AAAAAAAAAACAGGAGAAAGCGTAAGTGCCCTGACAAAGAAAAGAGGTAA |
rs7833904 P1-T | ALD-AAAAAAAAAACAGGAGAAAGCGTAAGTGCCCTGACAAAGAAAAGAGGTAT |
rs7833904 P2 | Phosphate-AGTTGCCAGAAAACCTGGCA-biotin |
rs7833904 Target for P1-A | TGCCAGGTTTTCTGGCAACTTTACCTCTTTTCTTTGTCAGG |
rs7833904 Target for P1-T | TGCCAGGTTTTCTGGCAACTATACCTCTTTTCTTTGTCAGG |
rs7833904 Forward | AATGTGAGCAAGAAAGCCC |
rs7833904 Reverse | GAACTCAGTTTATTTTGCCAGG |
rs1125226 P1-A | ALD-AAAAAAAAAACAACTGGTTCATCAGACTTGCTGCGACCAATTAACCTTGA |
rs1125226 P1-C | ALD-AAAAAAAAAACAACTGGTTCATCAGACTTGCTGCGACCAATTAACCTTGC |
rs1125226 P2 | Phosphate-CAGCTCAGGGAGAGAGAGAG-biotin |
rs1125226 Target for P1-A | CTCTCTCTCTCCCTGAGCTGTCAAGGTTAATTGGTCGCAGC |
rs1125226 Target for P1-C | CTCTCTCTCTCCCTGAGCTGGCAAGGTTAATTGGTCGCAGC |
rs1125226 Forward | TTTCTCTCTCTCCCTCTTTCTC |
rs1125226 Reverse | CTGGTTCATCAGACTTGCTG |
rs1023652 P1-C | ALD-AAAAAAAAAAAAAATTCAAGTACCATAATATTCACCCCTTTAAAGAGTAC |
rs1023652 P1-G | ALD-AAAAAAAAAAAAAATTCAAGTACCATAATATTCACCCCTTTAAAGAGTAG |
rs1023652 P2 | Phosphate-AATTCAAAATTTCTAGCATA-biotin |
rs1023652 Target for P1-C | TATGCTAGAAATTTTGAATTGTACTCTTTAAAGGGGTGAAT |
rs1023652 Target for P1-G | TATGCTAGAAATTTTGAATTCTACTCTTTAAAGGGGTGAAT |
rs1023652 Forward | TGGAATGCCAGTTACCCCAC |
rs1023652 Reverse | GGTGATGGTCACACAATCTTTG |
1302 delTT P1-TT | ALD-AAAAAAAAAATGTATTTTTATTGCAGACTTTTAAATATGATAGGTATCTT |
1302 delTT P1-DEL | ALD-AAAAAAAAAATTTGTATTTTTATTGCAGACTTTTAAATATGATAGGTATC |
1302 delTT P2 | Phosphate-GATGAAAACGGGAAGACAAA-biotin |
1302 delTT Target for P1-TT | TGTCTTCCCGTTTTCATCAAGATACCTATCATATTTAAAA |
1302 delTT Target for P1-DEL | TGTCTTCCCGTTTTCATCGATACCTATCATATTTAAAA |
1302 delTT Forward | ACTAGCTTAAAGGCGGTTTTC |
1302 delTT Reverse | CGATCCAAAGGGCATGTAG |