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Table 1 Primers for PCR-SSCP analysis of p66Shc gene

From: Search for genetic variants in the p66Shc longevity gene by PCR-single strand conformational polymorphism in patients with early-onset cardiovascular disease

Amplicon

Product size (bp)

Forward primer (5'-)

Reverse primer (5'-)

CH2

352

gcccctctttcacctcaact

atgtcctggaggaggggtag

Promoter-1

238

cagagaagaggctccacgtt

ggggcaggagatccatagtt

Promoter-2

247

cccacccccactttacttct

aacgtggagcctcctctctg

Promoter-3

330

aggacaggaagggaaatgct

agtgctgactcacaggctca

  1. Forward and reverse primer sequences are all 5'. Promoter-1 fragment starts at nucleotide -222 from start codon of exon1; Promoter-2 fragment starts at nucleotide -449 from start codon of exon1; Promoter-3 fragment starts at nucleotide -637 from start codon of exon1 of p66Shc gene.
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BMC Genomic Data

ISSN: 2730-6844

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