Primer combination | Specific primer (pmol) | Control primer | DMSO (%) | Annealing temperature (°C) | Extension time (sec) | ||
---|---|---|---|---|---|---|---|
HGH-F/R (pmol) | JK-L-F/R (pmol) | MO-21/31 (pmol) | |||||
Pk-5'-(-1056)-F | 5 | 1 | 64 | 40 | |||
Pk-5'-(-550)-R | 5 | ||||||
Pk-5'-(-1056)F | 5 | 1 | 64 | 40 | |||
Pk-5'-(-550insC)-R | 5 | ||||||
Pk-5'-(-834)-F | 7.5 | 0.5 | 4 | 62 | 60 | ||
Pk-5'-(-160A-mis)-R | 7.5 | ||||||
Pk-5'-(-834)-F | 5 | 0.5 | 3 | 62 | 60 | ||
Pk-5'-(-160G-mis)-R | 5 | ||||||
Pk-5'(-550insC)-F | 10 | 0.75 | 64 | 40 | |||
Pk-5'-(-160G)-R | 10 | 0.75 | |||||
Pk-5'(-550T)-F | 7.5 | 0.5 | 63 | 40 | |||
Pk-5'-(-160A)-R | 7.5 | 0.5 | |||||
Pk-109A-F or Pk-109G-F | 6 | 0.4 | 2 | 65 | 90 | ||
Pk-1697A-R or Pk-1697G-R* | 6 | ||||||
Pk-987A-F | 5 | 0.4 | 66 | 60 | |||
Pk-1697A-R | 5 | ||||||
Pk-987G-F | 5 | 0.4 | 66 | 60 | |||
Pk-1697G-R | 5 | ||||||
Pk-109A-F or Pk-109G-F | 7.5 | 0.5 | 64 | 60 | |||
Pk-1120-R** |