Figure 3
From: Epigenetic predisposition to expression of TIMP1 from the human inactive X chromosome
Nuclease sensitivity of TIMP1. Nuclei from various cell lines (as indicated on the left) were treated with increasing amounts of DNase I (0, 0.1, 0.25, 0.5, 1.0 units) as represented by the increasing breadth of the triangle. The DXS8037 primers flank a non-coding region and were used to check that equal digestion and PCR amplification occurred across all cell lines. The cell line designations indicate X activity, TIMP1 expression status, and methylation status as shown in Figure 2B.