Development of novel heminested PCR assays based on mitochondrial 16s rRNA gene for identification of seven pecora species

Table 2 Results from species-specific heminested PCR assay with target and non target species for designed primers

Species	Common Name	DNA source	1^st PCR universal primer 16s rRNA gene	2^nd PCR BB 16	2^nd PCR GL 16	2^nd PCR NG 16	2^nd PCR TH 16	2^nd PCR CH 16	2^nd PCR SM 16	2^nd PCR HD 16
Antilope cervicapra a	Blackbuck	Tissue	+	+	-	-	-	-	-	-
Antilope cervicapra b	Blackbuck	Tissue	+	+	-	-	-	-	-	-
Antilope cervicapra c	Blackbuck	Blood stain	+	+	-	-	-	-	-	-
Boselaphus tragocamelus a	Nilgai	Tissue	+	-	-	+	-	-	-	-
Boselaphus tragocamelus b	Nilgai	Tissue	+	-	-	+	-	-	-	-
Boselaphus tragocamelus c	Nilgai	Tissue	+	-	-	+	-	-	-	-
Naemorhedus goral a	Goral	Blood stain	+	-	+	-	-	-	-	-
Naemorhedus goral b	Goral	Tissue	+	-	+	-	-	-	-	-
Naemorhedus goral c	Goral	Tissue	+	-	+	-	-	-	-	-
Axis porcinus a	Hog deer	Tissue	+	-	-	-	-	-	-	+
Axis porcinus b	Hog deer	Tissue	+	-	-	-	-	-	-	+
Cervus axis a	Chital	Blood stain	+	-	-	-	-	+	-	-
Cervus axis b	Chital	Tissue	+	-	-	-	-	+	-	-
Cervus axis c	Chital	Tissue	+	-	-	-	-	+	-	-
Cervus axis d	Chital	Tissue	+	-	-	-	-	+	-	-
Cervus axis e	Chital	Tissue	+	-	-	-	-	+	-	-
Cervus eldi a	Thamin	Tissue	+	-	-	-	+	-	-	-
Cervus eldi b	Thamin	Tissue	+	-	-	-	+	-	-	-
Cervus unicolor a	Sambar	Tissue	+	-	-	-	-	-	+	-
Cervus unicolor b	Sambar	Tissue	+	-	-	-	-	-	+	-
Cervus duvaucelii	Barasingha	Tissue	+	-	-	-	-	-	-	-
Ovis aries	Sheep	Tissue	+	-	-	-	-	-	-	-
Bos tarus	Cow	Tissue	+	-	-	-	-	-	-	-
Homo sapiens	Human	Blood	+	-	-	-	-	-	-	-
Lepidochelys olevacea	Turtle	Tissue	+	-	-	-	-	-	-	-
Hemidactylus fluviviridis	Lizard	Tissue	+	-	-	-	-	-	-	-