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Table 1 CT values, ΔCT, and ΔΔCT

From: A novel replication-independent histone H2a gene in mouse

Time (h)

GAPDH

E130307C13_1

ΔCT

ΔΔCT

Expression

E130307C13_2

ΔCT

ΔΔCT

Expression

Hist2h2aa_1

ΔCt

ΔΔCT

Expression

Hist2h2aa_2

ΔCT

ΔΔCT

Expression

H2afz

ΔCT

ΔΔCT

Expression

0

14.4

19.1

4.65

0

1

20

5.61

0

1

18.1

3.63

0

1

19.3

4.9

0

1

16.8

2.35

0

1

1

14.4

19

4.64

-0.01

1.01

20.1

5.71

0.1

0.93

17.5

3.1

-0.53

1.44

19.2

4.79

-0.11

1.08

16.6

2.26

-0.09

1.06

2

14.4

18.9

4.54

-0.11

1.08

19.7

5.32

-0.29

1.22

16.8

2.42

-1.21

2.31

18.3

3.92

-0.98

1.97

16.7

2.33

-0.02

1.01

3

14.3

18.8

4.5

-0.15

1.11

19.7

5.4

-0.21

1.16

16.8

2.48

-1.15

2.22

18.3

3.92

-0.98

1.97

16.8

2.46

0.11

0.93

4

14.4

19.2

4.78

0.13

0.91

19.9

5.49

-0.12

1.09

17.2

2.79

-0.84

1.79

18.3

3.93

-0.97

1.96

16.8

2.43

0.08

0.95

5

14.4

19.2

4.82

0.17

0.89

20

5.61

0

1

17.1

2.66

-0.97

1.96

18.6

4.17

-0.73

1.66

16.8

2.42

0.07

0.95

6

14.1

19.8

5.69

1.04

0.49

20.4

6.33

0.72

0.61

18.4

4.29

0.66

0.63

19.9

5.8

0.9

0.54

16.8

2.71

0.36

0.78

7

14.2

19.7

5.45

0.8

0.57

20.3

6.11

0.5

0.71

17.6

3.35

-0.28

1.21

19

4.83

-0.07

1.05

16.9

2.7

0.35

0.78

8

14.3

19.2

4.87

0.22

0.86

20.1

5.71

0.1

0.93

17.6

3.3

-0.33

1.26

19.2

4.81

-0.09

1.06

16.9

2.55

0.2

0.87

9

14.3

19.3

5.03

0.38

0.77

20

5.76

0.15

0.9

17.8

3.54

-0.09

1.06

19.3

5.01

0.11

0.93

16.6

2.34

-0.01

1.01

10

14.2

19.1

4.83

0.18

0.88

19.8

5.58

-0.03

1.02

17.8

3.6

-0.03

1.02

18.7

4.5

-0.4

1.32

16.6

2.4

0.05

0.97

11

14.3

19.8

5.5

0.85

0.55

20.2

5.87

0.26

0.84

18.3

4

0.37

0.77

19.2

4.91

0.01

0.99

16.2

1.94

-0.41

1.33