Table 7 Primer sequences used for the amplification of the Cftr intron 9-pMC1 vector plasmid sequence (CFneo2) and the neomycin-Cftr intron 9 (CFneo1) products.
From: Instability of the insertional mutation in CftrTgH(neoim)Hgucystic fibrosis mouse model
Primer name | Primer sequence 5'-3' | Expected size |
|---|---|---|
CFneo 1-A | CGT TGG CTA CCC GTG ATA TT | 332 bp |
CFneo 1-B | CTT CCA CAA GGC TTC CTG AG | |
CFneo2-A | CCT GAT GTT GAT TTT GGG AGA | 253 bp |
CFneo2-B | ATT AAT GCA GCT GGC ACG AC |