Table 6 Primer sequences used for the amplification of the intragenic Cftr microsatellites. The forward primer is 5'biotinylated.
From: Instability of the insertional mutation in CftrTgH(neoim)Hgucystic fibrosis mouse model
Primer name | Primer sequence 5'-3' |
|---|---|
D6NC1-A | BIOTIN-TGC TTG AGC TAT CCA TTC TGA |
D6NC1-B | TAC CCA ATG TTG CCA TCT GA |
D6NC2-A | BIOTIN-TTG GAA GTG AGG ATT GCC TT |
D6NC2-B | TGC CTC AGT CTC ATA TTA TTG C |
D6NC3-A | BIOTIN-TCT CAG CCT GTC TTC CTC TCA |
D6NC3-B | TCC TCC CAA AAC AGC TTC AC |
D6NC4-A | BIOTIN-GAG TTG GAG AGG CTG TTT GG |
D6NC4-B | TGT GCC AGG ACA CTG TGA CT |
D6NC5-A | BIOTIN-TTC AAA TGA CCA AAA TCC CC |
D6NC5-B | TGG CAA ATT TTC AAC AAC AAA |