Table 3 Primer sequences used for the amplification of the long range products.
From: Instability of the insertional mutation in CftrTgH(neoim)Hgucystic fibrosis mouse model
Primer name | Primer sequence 5'-3' | Expected size | Allele |
|---|---|---|---|
Cftr-5012 | CCT TCC ATG TAC CCC TCC TCA CTT | 5012 bp | Wild type |
CCC GGC ATA ATC CAA GAA AAT TG | |||
Cftr-5198 | TGT GGG AAA TCC TGT GCT GAA A | 5198 bp | mutant |
CTT CCG GCT CGT ATG TTG TGT T | |||
Cftr-3736 | CAC ACA ACA TAC GAG CCG GAA G | 3736 bp | mutant |
TTT ATT GCC GAT CCC CTC AGA A | |||
Cftr-3473 | CTC GTG CTT TAC GGT ATC GCC | 3473 bp | mutant |
TGC TGT AGT TGG CAA GCT TTG A |