TY - JOUR AU - Charizopoulou, Nikoletta AU - Jansen, Silke AU - Dorsch, Martina AU - Stanke, Frauke AU - Dorin, Julia R. AU - Hedrich, Hans-Jürgen AU - Tümmler, Burkhard PY - 2004 DA - 2004/04/21 TI - Instability of the insertional mutation in CftrTgH(neoim)Hgucystic fibrosis mouse model JO - BMC Genetics SP - 6 VL - 5 IS - 1 AB - A major boost to the cystic fibrosis disease research was given by the generation of various mouse models using gene targeting in embryonal stem cells. Moreover, the introduction of the same mutation on different inbred strains generating congenic strains facilitated the search for modifier genes. From the original CftrTgH(neoim)HguCF mouse model we have generated using strict brother × sister mating two inbred CftrTgH(neoim)Hgumouse lines (CF/1 and CF/3). Thereafter, the insertional mutation was introgressed from CF/3 into three inbred backgrounds (C57BL/6, BALB/c, DBA/2J) generating congenic animals. In every backcross cycle germline transmission of the insertional mutation was monitored by direct probing the insertion via Southern RFLP. In order to bypass this time consuming procedure we devised an alternative PCR based protocol whereby mouse strains are differentiated at the Cftr locus by Cftr intragenic microsatellite genotypes that are tightly linked to the disrupted locus. SN - 1471-2156 UR - https://doi.org/10.1186/1471-2156-5-6 DO - 10.1186/1471-2156-5-6 ID - Charizopoulou2004 ER -