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Table 2 Number of cells/colony (fitness) of de3-01-CG Ade+ revertants (1–17). We estimated the colonies' fitness as the number of cells per colony 30 on SDNA+ade as well as on YEPD after four days of incubation at 28°C. The strain de3-01-CG was used as control; see "Methods" for details.

From: The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine

Strain/Revertant

No. of cells /colony (×107) on:

Fitness ratio SDNA+ade/YEPD

 

YEPD

SDNA+ade

 

de3-01-CG

4.380

1.700

0.388

1

1.700

-a

-a

2

0.070

-a

-a

3

-b

-b

-b

4

2.300

0.040

0.017

5

3.300

0.079

0.024

6

0.200

0.026

0.130

7

0.870

0.026

0.030

8

0.730

0.200

0.274

9

0.260

-a

-

10

3.900

0.200

0.051

11

1.700

0.300

0.176

12

1.700

0.210

0.124

13

2.600

0.100

0.038

14

1.600

0.200

0.125

15

0.700

0.080

0.114

16

0.500

0.080

0.160

17

1.700

0.210

0.124

  1. a Hardly visible at the stereomicroscope after 4 days of incubation at 28°C, diameter not estimable.
  2. b Not yet visible at the stereomicroscope after 4 days of incubation at 28°C. Visible colonies developed by day 8.