Skip to main content

Table 2 Number of cells/colony (fitness) of de3-01-CG Ade+ revertants (1–17). We estimated the colonies' fitness as the number of cells per colony 30 on SDNA+ade as well as on YEPD after four days of incubation at 28°C. The strain de3-01-CG was used as control; see "Methods" for details.

From: The exceptionally high rate of spontaneous mutations in the polymerase delta proofreading exonuclease-deficient Saccharomyces cerevisiae strain starved for adenine

Strain/Revertant No. of cells /colony (×107) on: Fitness ratio SDNA+ade/YEPD
  YEPD SDNA+ade  
de3-01-CG 4.380 1.700 0.388
1 1.700 -a -a
2 0.070 -a -a
3 -b -b -b
4 2.300 0.040 0.017
5 3.300 0.079 0.024
6 0.200 0.026 0.130
7 0.870 0.026 0.030
8 0.730 0.200 0.274
9 0.260 -a -
10 3.900 0.200 0.051
11 1.700 0.300 0.176
12 1.700 0.210 0.124
13 2.600 0.100 0.038
14 1.600 0.200 0.125
15 0.700 0.080 0.114
16 0.500 0.080 0.160
17 1.700 0.210 0.124
  1. a Hardly visible at the stereomicroscope after 4 days of incubation at 28°C, diameter not estimable.
  2. b Not yet visible at the stereomicroscope after 4 days of incubation at 28°C. Visible colonies developed by day 8.