Detection of allelic variations of human gene expression by polymerase colonies

BMC Genomic Data

Table 1 The relative expression of 4208A, 4208G PKD2 alleles using polony technology.

Ratio of 4208A/4208G PKD2 alleles in cDNA
	# of polonies			G/A ratio
Individual	4208A	4208G	This Study	P-value	Yan et al.
49	186	236	1.27	0.0149	1.2
50	152	267	1.76	1.94 × 10^-8	1.85
50-3	256	364	1.42	1.44 × 10^-5	1.72
50-4	112	113	1.01	0.947	0.95
50-5	385	571	1.48	1.80 × 10^-9	1.81
50-6	337	246	0.73	1.64 × 10^-4	0.82
50-7	222	197	0.89	0.222	0.95
50-12	96	96	1.00	1.000	1.05

For all patients, the polony count for both alleles represents the total number of polonies for the respective species observed in quadruplicate gels following polony analysis of cDNA. The P-value indicates the probability that the alleles are expressed at an equal ratio given the data. The P-value indicates that significance of the result.

ISSN: 2730-6844