Figure 4

Expression profile of ZmPox3. Total RNA was isolated from the indicated tissues and amplified by RT-PCR. Amplification products were visualized by agarose gel electrophoresis. RNA transcribed from plasmid pAW109 was used as an external loading control. The ZmPox3 forward primer (U19S1) was combined with a reverse primer upstream (U19R1) or downstream (U19MITEAS) of the MITE insertion. PCR cycles used to evaluate ZmPox3 varied between panel A and panel B (indicated by numbers to the right of the gel pictures). A) ZmPox3 and AldOMT expression in lines F7012 (mutant allele) or Lan496 (normal allele). The stem with the leaf sheaths of 50 day old plants was divided into a basal part (4 inter-nodes) and an upper part (ending at the base of the tassel). In addition a 30 cm long leaf blade was harvested. B) ZmPox3 and AldOMT expression in different parts of line F2 (normal allele). At flowering the stem with the leaf sheaths was cut at the base of every node yielding units of node (N) and adjacent inter-node (i) with numbering starting at the base of the plant. In addition the ear leaves were harvested at defined stages expressed in days after pollination (DAP).