Table 1 PCR primers used in PCR-SSCP analysis to identify heterozygosity in genomic DNA samples from abortuses.
From: Paternal imprinting of the SLC22A1LS gene located in the human chromosome segment 11p15.5
Sl. No. | Primer set | Sequence | Amplicon size |
|---|---|---|---|
1. | BWR3F | 5'-AAGGCGACAGTTCTGCCGGATGA-3' | 212 bp |
| Â | BWR3R | 5'-CAAGCCTGGGCTCACCTCTAAG-3' | Â |
2. | BWR4F | 5'-GCCCCACTGTGATGGACAAGCCTA-3' | 259 bp |
| Â | 22A1LSR | 5'-AGTGGGCGGCGGGACCCGTTTTG-3' | Â |
3. | 22F | 5'-GTACAGCGTTCCTGACAACGTTC-3' | 267 p |
| Â | 22R | 5'-GTGCTAGTGCCCTAAATCCTGCA-3' | Â |