Figure 5

Rad26-GFP spots and speckles form after Bleomycin treatment. Cultures were grown at 30°C in liquid, complete media to O.D. 0.3 and then treated with 5 mU/ml of Bleomycin for 3 hours. Cells were then prepared for microscopy following the Triton X-100 extraction method (see Methods). A. Rad26-GFP speckles in Bleomycin-treated rad26-GFP (TE1197) cells. B. Rad26-GFP spots and speckles in Bleomycin-treated rad26-GFP cells. The arrow points to the background of speckles in this cell that contains one bright spot. C. Rad26-GFP spots and speckles in Bleomycin-treated rad9Δrad26-GFP (TE1193) cells. The arrow points to the background of speckles in this cell that contains two spots. Bar = 5 μm