hus1Δ and rad17Δ are not sensitive to TBZ. Cultures were grown at 30°C in liquid, complete media to 1 × 106 cells/ml. Two-fold serial dilutions of cultures were made onto complete agar media (YE5S) and complete agar media containing 20 μg/ml TBZ (20 μg/ml) and incubated at 30°C. Pictures of YE5S plates were taken after two days of growth, while those of YE5S + 20 μg/ml TBZ plates were taken after four days. Wildtype (TE696), hus1Δ (TE484), rad9Δ (TE794), rad17Δ (TE864), rad1Δ (TE459) and rad26Δ (TE257).