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Table 1 Rabbit and Human MYOC primers used for PCR and Cloning

From: Characterization of rabbit myocilin: Implications for human myocilin glycosylation and signal peptide usage

Primer Sequence
Exon1F* 5'-CAGTCCCAATGAATCCAGC-3'
Exon1R 5'-CTCTCTGGTTTGGGTTTCCA-3'
5'RACE 5'-CAGCTCCTTCTGCAATTCCTCCT-3'
5'RACEnest 5'-CTTCTGCAATTCCTCCTGGATCTC-3'
3'RACE 5'-CATCCAGGACCTCCAGAGAGACAG-3'
3'RACEnest 5'-ACCTCCAGAGAGACAGTACCC-3'
P355F 5'-CTTCTCAATGGAATTTGGACACTT-3'
P356F 5'-GATGTGGGGAACTAGTTTGGGTAG-3'
P356R 5'-AGTGCGGCCGC TTAAATCTTGGACAGCTTGATGTC-3'
P357F 5'-GCAGGATCC CCAAGGTTTTTCCCTGCACATTGCTGC-3'; BamH1 underlined
P365R 5'-AGTGCGGCCGC GTTGTCTCCCTCTTCACCC-3'; Not1 underlined
P368R 5'-pCTTCCCTGGAGCCTGCAGGCACAT-3'; p indicates phosphorylation
P370R 5'-pCGTTGTCTCCCTCTTCACCCCTGG-3'; p indicates phosphorylation
P235 5'-CCCAAGCTT ATGCCAGCTGTCCAGCTGCT-3'; HindIII underlined
P236 5'-CCCAAGCTT ATGAGGACAGCTCAGCTCAGGAAG-3'; HindIII underlined
P237 5'-CCGCCTCAGG GTGCCCAGCT-3'; Bsu36I underlined
P316F 5'-GGCCAGTCCCAG TGAATCCAGCTGCCC-3'; Mutation underlined
P316R 5'-GGGCAGCTGGATTCAC TGGGACTGGCC-3'; Mutation underlined
  1. * Note: F, forward orientation; R, reverse orientation