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Figure 3 | BMC Genetics

Figure 3

From: Characterization of rabbit myocilin: Implications for human myocilin glycosylation and signal peptide usage

Figure 3

Rabbit MYOC expression. Western immunoblot analysis of myocilin in (A) rabbit, human, and monkey aqueous humor samples treated with (+) or without (-) glycosidases and in (B) GTM66 cells transfected with expression vectors encoding wild-type human MYOC (WT), N-terminal 14 (ΔN14) or 32 (ΔN32) aa deleted human MYOC, or N57S mutated human MYOC. I, intracellular myocilin present in the cell lysate; E, extracellular myocilin secreted into the medium. 57/55-kDa myocilin doublet is indicated with arrows.

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